
Originally Posted by
Emil Martinec
That's a question of where your exposure lies. For a fixed exposure (Tv and Av) ISO 800 will have less noise (mostly in shadows) than ISO 640 (which is as noisy as 400 underexposed on 1 series prior to the 1D4, and perhaps also the latter if and when it is tested for read noise). So the real question is whether you are free to open up the exposure to the point where ISO 800 would lose you highlights that you want to keep. If not, use 800; if so, then use a lower ISO.
For a fixed exposure, you are always getting the same number of photons; what changes with ISO is that the number of photons that corresponds to saturation of the raw data drops in proportion to ISO, and as a secondary effect the read noise goes down relative to a fixed signal as the ISO increases. This fact is about the only reason there needs to be and ISO setting in the camera, rather than just a metadata tag saying what the metering was, so that the converter could apply an appropriate signal boost before doing the conversion. Since the read noise goes down with ISO in terms of photon equivalents, it pays to boost the ISO in the camera for weak photon signals (low light).